3 3 diaminobenzidine dab solution (Vector Laboratories)

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Vector Laboratories 3 3 diaminobenzidine dab solution
3 3 Diaminobenzidine Dab Solution, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 12433 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/3+3+diaminobenzidine+dab+solution/DAB+Peroxidase+(HRP)+Substrate+Kit+(with+Nickel)%2C+3%2C3%E2%80%99-diaminobenzidine/pm42010671-105-24-28
Average 96 stars, based on 12433 article reviews

3 3 diaminobenzidine dab solution - by Bioz Stars, 2026-10

96/100 stars

Images

Staining:

Article Title: ALDH2 protects against dopaminergic neuronal cell ferroptosis by enhancing the enzyme activity of PRDX6 in Parkinson’s disease
Article Snippet: The brain slices were transferred to secondary antibody (1:200, Vector Laboratories) for 1 h at 37 °C and then to AB peroxidase (1:200, Vector Laboratories) for 45 min on the second day. .. Staining was visualized using 3,3′-diaminobenzidine (DAB) solution (Vector Laboratories). .. Using a GSH assay kit (Nanjing, Jiancheng A006-1-1), the solution and standards were prepared and stored under specified conditions according to the manufacturer’s instructions.

Incubation:

Article Title: Multi-layer stratified oncology platform utilizing transcriptomics, prostate cancer organoids, and modeling of drug response
Article Snippet: The primary antibody targeting MET Tyr1234/5 (1:150, clone D26, CST, 3077; RRID: AB_2143884) was added overnight at + 4 °C. .. The next day, the visualization was achieved through the consecutive incubation of the secondary antibody goat biotinylated anti-rabbit IgG (Vector Laboratories, PK-6101) for 1 h, the ABC reagent solution (Vector Laboratories, PK-6101) for 30 min, and the 3,3’-diaminobenzidine (DAB) solution (Vector Laboratories, SK-4100) for 10 min. Hematoxylin was used for counterstaining and the Eukitt mounting medium (Sigma-Aldrich, 03989) to cover the samples. .. The slides were scanned with a Pannoramic 250 Flash III Scanner (3DHISTECH).

Article Title: Sex- and cell type-specific effects of dexmedetomidine on ferroptosis in neurons and microglia following traumatic brain injury in juvenile mice.
Article Snippet: .. The sections were then rinsed in deionized water and incubated for 15 min in 3,3’-diaminobenzidine (DAB) solution (Vector Laboratories, CA, USA). .. After wash, the sections were incubated overnight with Guinea pig anti-NeuN (a neuronal marker; 1:250; Cat# ABN90, MilliporeSigma, MA.

Article Title: Sex- and cell type-specific effects of dexmedetomidine on ferroptosis in neurons and microglia following traumatic brain injury in juvenile mice
Article Snippet: .. The sections were then rinsed in deionized water and incubated for 15 min in 3,3’-diaminobenzidine (DAB) solution (Vector Laboratories, CA, USA). .. After wash, the sections were incubated overnight with Guinea pig anti-NeuN (a neuronal marker; 1:250; Cat# ABN90, MilliporeSigma, MA.

Microscopy:

Article Title: Immunohistochemical analysis to detect a molecular signature in intervertebral disc degeneration.
Article Snippet: For immunohistochemical evaluation, sections were incubated overnight (4 °C) with a primary antibody against FOXO3a (#ab70315, rabbit anti-human, 1:100 dilution; Abcam, Cambridge, UK), SOD2 (#sc-133134, mouse anti-human, 1:100 dilution; Santa Cruz biotech., Dallas, USA), HIF1α (clone H1alpha67, mouse anti-human, 1:200 dilution; Novusbio, Centennial, USA), GLUT1 (#TA301678, mouse antihuman, 1:200 dilution; Origene, Rockville, USA), and BRY (#ab209665, rabbit anti-human, 1:500 dilution; Abcam), followed by treatment with Vectastain ABC solution (#MP7500; Vectorlabs, Burlingame, USA) for 30 min. To achieve specificity, the working concentrations of primary antibodies were selected by following the dilution guidelines and technical specifications provided by the antibody’s manufacturer. .. Reactions were developed using 3,3′-diaminobenzidine (DAB) solution (Vectorlabs), sections were counterstained with hematoxylin, mounted in glycerol and observed under a bright-field microscope (Nikon Eclipse 50i; objectives: Nikon CFI Achr Adl 10× Ph1 Na 0.25 #MRP40102, Nikon CFI Achr Adl 40× Ph1 Na 0.55 #MRP46402; Nikon Corporation, Tokyo, Japan). .. For immunohistochemistry, images were acquired using a CMOS camera with an IMX485 image sensor (Sony, Tokyo, Japan), 8.3-megapixel with a native resolution of 3840 × 2160 pixels.

Article Title: Immunohistochemical analysis to detect a molecular signature in intervertebral disc degeneration
Article Snippet: For immunohistochemical evaluation, sections were incubated overnight (4 °C) with a primary antibody against FOXO3a (#ab70315, rabbit anti-human, 1:100 dilution; Abcam, Cambridge, UK), SOD2 (#sc-133134, mouse anti-human, 1:100 dilution; Santa Cruz biotech., Dallas, USA), HIF1α (clone H1alpha67, mouse anti-human, 1:200 dilution; Novusbio, Centennial, USA), GLUT1 (#TA301678, mouse anti-human, 1:200 dilution; Origene, Rockville, USA), and BRY (#ab209665, rabbit anti-human, 1:500 dilution; Abcam), followed by treatment with Vectastain ABC solution (#MP-7500; Vectorlabs, Burlingame, USA) for 30 min. To achieve specificity, the working concentrations of primary antibodies were selected by following the dilution guidelines and technical specifications provided by the antibody’s manufacturer. .. Reactions were developed using 3,3′-diaminobenzidine (DAB) solution (Vectorlabs), sections were counterstained with hematoxylin, mounted in glycerol and observed under a bright-field microscope (Nikon Eclipse 50i; objectives: Nikon CFI Achr Adl 10× Ph1 Na 0.25 #MRP40102, Nikon CFI Achr Adl 40× Ph1 Na 0.55 #MRP46402; Nikon Corporation, Tokyo, Japan). .. For immunohistochemistry, images were acquired using a CMOS camera with an IMX485 image sensor (Sony, Tokyo, Japan), 8.3-megapixel with a native resolution of 3840 × 2160 pixels.

Expressing:

Article Title: Deficiency of GCN5 exacerbates pulmonary fibrosis by disrupting the LKB1-AMPK pathway.
Article Snippet: After blocking, the slides were incubated with the following primary antibodies: anti-GCN5 (sc-365321; Santa Cruz Biotechnology, USA) and anti-αSMA (ab5694; Abcam, UK). .. The expression level was identified using the HRPconjugated secondary antibody (K5007; Dako, USA) for 1 h at room temperature, followed by 1 min of 3,3’-Diaminobenzidine (DAB) solution (SK-4100; Vectorlab, USA) and hematoxylin counterstaining. ..

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